For me, it is when they make guys look like freaking chicks and I can never tell which one they are. This goes for Haku from Naruto and some guy from Sengoku Basara whose name I forgot.
EDIT: Also, chicks with extremely oversized breasts.
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Edited by God: 8/14/2013 7:12:39 PMOverused plot lines, particularly the "random guy suddenly lives with some supernatural girl who fights off people constantly trying to capture her."